Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1334579..1335273 Replicon chromosome
Accession NZ_CP122454
Organism Escherichia coli strain 82.0311

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag QBY76_RS06335 Protein ID WP_001263500.1
Coordinates 1334875..1335273 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag QBY76_RS06330 Protein ID WP_000554758.1
Coordinates 1334579..1334872 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QBY76_RS06305 (1330017) 1330017..1330514 + 498 WP_000006241.1 REP-associated tyrosine transposase RayT -
QBY76_RS06310 (1330509) 1330509..1330853 - 345 WP_124072110.1 hypothetical protein -
QBY76_RS06315 (1330932) 1330932..1332644 - 1713 Protein_1232 flagellar biosynthesis protein FlhA -
QBY76_RS06320 (1332616) 1332616..1333401 + 786 WP_061336152.1 putative lateral flagellar export/assembly protein LafU -
QBY76_RS06325 (1333472) 1333472..1334527 + 1056 WP_001226168.1 DNA polymerase IV -
QBY76_RS06330 (1334579) 1334579..1334872 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QBY76_RS06335 (1334875) 1334875..1335273 + 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QBY76_RS06340 (1335283) 1335283..1335735 + 453 WP_001059895.1 GNAT family N-acetyltransferase -
QBY76_RS06345 (1335925) 1335925..1337064 + 1140 WP_000521577.1 RNA ligase RtcB family protein -
QBY76_RS06350 (1337061) 1337061..1337675 + 615 WP_000602123.1 peptide chain release factor H -
QBY76_RS06355 (1337732) 1337732..1339189 - 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
QBY76_RS06360 (1339450) 1339450..1339908 + 459 WP_001291988.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T276923 WP_001263500.1 NZ_CP122454:1334875-1335273 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT276923 WP_000554758.1 NZ_CP122454:1334579-1334872 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References