Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4005736..4006430 Replicon chromosome
Accession NZ_CP122451
Organism Escherichia coli strain 82.0312

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag QBY78_RS19655 Protein ID WP_001263500.1
Coordinates 4005736..4006134 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag QBY78_RS19660 Protein ID WP_000554758.1
Coordinates 4006137..4006430 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QBY78_RS19630 (4001101) 4001101..4001559 - 459 WP_001291988.1 xanthine phosphoribosyltransferase -
QBY78_RS19635 (4001820) 4001820..4003277 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
QBY78_RS19640 (4003334) 4003334..4003948 - 615 WP_000602123.1 peptide chain release factor H -
QBY78_RS19645 (4003945) 4003945..4005084 - 1140 WP_000521577.1 RNA ligase RtcB family protein -
QBY78_RS19650 (4005274) 4005274..4005726 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
QBY78_RS19655 (4005736) 4005736..4006134 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QBY78_RS19660 (4006137) 4006137..4006430 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QBY78_RS19665 (4006482) 4006482..4007537 - 1056 WP_001226168.1 DNA polymerase IV -
QBY78_RS19670 (4007608) 4007608..4008393 - 786 WP_061336152.1 putative lateral flagellar export/assembly protein LafU -
QBY78_RS19675 (4008365) 4008365..4010077 + 1713 Protein_3862 flagellar biosynthesis protein FlhA -
QBY78_RS19680 (4010156) 4010156..4010500 + 345 WP_124072110.1 hypothetical protein -
QBY78_RS19685 (4010495) 4010495..4010992 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T276911 WP_001263500.1 NZ_CP122451:c4006134-4005736 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT276911 WP_000554758.1 NZ_CP122451:c4006430-4006137 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References