Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2548989..2549551 Replicon chromosome
Accession NZ_CP122451
Organism Escherichia coli strain 82.0312

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag QBY78_RS12175 Protein ID WP_000605675.1
Coordinates 2548989..2549267 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag QBY78_RS12180 Protein ID WP_000781370.1
Coordinates 2549267..2549551 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QBY78_RS12150 (2544575) 2544575..2545006 - 432 WP_000152305.1 peroxiredoxin OsmC -
QBY78_RS12155 (2545352) 2545352..2545567 + 216 WP_000495766.1 biofilm-dependent modulation protein -
QBY78_RS12160 (2545669) 2545669..2545806 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
QBY78_RS12165 (2545962) 2545962..2547659 + 1698 WP_000433462.1 malate dehydrogenase -
QBY78_RS12170 (2547793) 2547793..2548803 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
QBY78_RS12175 (2548989) 2548989..2549267 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QBY78_RS12180 (2549267) 2549267..2549551 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
QBY78_RS12185 (2549780) 2549780..2550433 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
QBY78_RS12190 (2550426) 2550426..2551310 - 885 WP_001240581.1 formate dehydrogenase N subunit beta -
QBY78_RS12195 (2551323) 2551323..2554370 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T276908 WP_000605675.1 NZ_CP122451:2548989-2549267 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT276908 WP_000781370.1 NZ_CP122451:2549267-2549551 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References