Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3900806..3901500 Replicon chromosome
Accession NZ_CP122448
Organism Escherichia coli strain 85.0412

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag QBY77_RS18870 Protein ID WP_001263500.1
Coordinates 3900806..3901204 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag QBY77_RS18875 Protein ID WP_000554758.1
Coordinates 3901207..3901500 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QBY77_RS18845 (3896171) 3896171..3896629 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
QBY77_RS18850 (3896890) 3896890..3898347 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
QBY77_RS18855 (3898404) 3898404..3899018 - 615 WP_000602124.1 peptide chain release factor H -
QBY77_RS18860 (3899015) 3899015..3900154 - 1140 WP_000521561.1 RNA ligase RtcB family protein -
QBY77_RS18865 (3900344) 3900344..3900796 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
QBY77_RS18870 (3900806) 3900806..3901204 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QBY77_RS18875 (3901207) 3901207..3901500 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QBY77_RS18880 (3901552) 3901552..3902607 - 1056 WP_001226168.1 DNA polymerase IV -
QBY77_RS18885 (3902678) 3902678..3903463 - 786 WP_000207578.1 putative lateral flagellar export/assembly protein LafU -
QBY77_RS18890 (3903435) 3903435..3905147 + 1713 Protein_3704 flagellar biosynthesis protein FlhA -
QBY77_RS18895 (3905175) 3905175..3905384 + 210 WP_071778446.1 hypothetical protein -
QBY77_RS18900 (3905467) 3905467..3905964 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3900806..3921160 20354


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T276882 WP_001263500.1 NZ_CP122448:c3901204-3900806 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT276882 WP_000554758.1 NZ_CP122448:c3901500-3901207 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References