Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3599941..3600620 Replicon chromosome
Accession NZ_CP122448
Organism Escherichia coli strain 85.0412

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QBY77_RS17460 Protein ID WP_000057523.1
Coordinates 3600318..3600620 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QBY77_RS17455 Protein ID WP_000806442.1
Coordinates 3599941..3600282 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QBY77_RS17445 (3596185) 3596185..3597117 - 933 WP_000883025.1 glutaminase A -
QBY77_RS17450 (3597379) 3597379..3599883 + 2505 WP_000083943.1 copper-exporting P-type ATPase CopA -
QBY77_RS17455 (3599941) 3599941..3600282 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QBY77_RS17460 (3600318) 3600318..3600620 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QBY77_RS17465 (3600753) 3600753..3601547 + 795 WP_000365148.1 TraB/GumN family protein -
QBY77_RS17470 (3601751) 3601751..3602230 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QBY77_RS17475 (3602254) 3602254..3603054 + 801 WP_000439798.1 hypothetical protein -
QBY77_RS17480 (3603051) 3603051..3603554 + 504 WP_000667000.1 hypothetical protein -
QBY77_RS17485 (3603592) 3603592..3605244 - 1653 WP_000771760.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T276879 WP_000057523.1 NZ_CP122448:c3600620-3600318 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT276879 WP_000806442.1 NZ_CP122448:c3600282-3599941 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References