Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2644505..2645067 Replicon chromosome
Accession NZ_CP122448
Organism Escherichia coli strain 85.0412

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag QBY77_RS12700 Protein ID WP_000605675.1
Coordinates 2644505..2644783 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag QBY77_RS12705 Protein ID WP_000781370.1
Coordinates 2644783..2645067 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QBY77_RS12675 (2640091) 2640091..2640522 - 432 WP_000152305.1 peroxiredoxin OsmC -
QBY77_RS12680 (2640867) 2640867..2641082 + 216 WP_000495766.1 biofilm-dependent modulation protein -
QBY77_RS12685 (2641184) 2641184..2641321 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
QBY77_RS12690 (2641478) 2641478..2643175 + 1698 WP_000433462.1 malate dehydrogenase -
QBY77_RS12695 (2643309) 2643309..2644319 + 1011 WP_000642380.1 alcohol dehydrogenase AdhP -
QBY77_RS12700 (2644505) 2644505..2644783 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QBY77_RS12705 (2644783) 2644783..2645067 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
QBY77_RS12710 (2645296) 2645296..2645949 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
QBY77_RS12715 (2645942) 2645942..2646826 - 885 WP_001240581.1 formate dehydrogenase N subunit beta -
QBY77_RS12720 (2646839) 2646839..2649886 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T276878 WP_000605675.1 NZ_CP122448:2644505-2644783 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT276878 WP_000781370.1 NZ_CP122448:2644783-2645067 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References