Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3958102..3958699 Replicon chromosome
Accession NZ_CP122389
Organism Klebsiella pneumoniae strain KP9650

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag QCB43_RS19480 Protein ID WP_004142563.1
Coordinates 3958382..3958699 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag QCB43_RS19475 Protein ID WP_004142561.1
Coordinates 3958102..3958389 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QCB43_RS19445 (QCB43_19445) 3954182..3954430 + 249 WP_002893037.1 DUF1158 domain-containing protein -
QCB43_RS19450 (QCB43_19450) 3954448..3954789 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
QCB43_RS19455 (QCB43_19455) 3954820..3955935 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
QCB43_RS19460 (QCB43_19460) 3956115..3956696 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
QCB43_RS19465 (QCB43_19465) 3956696..3957064 + 369 WP_279510798.1 MmcQ/YjbR family DNA-binding protein -
QCB43_RS19470 (QCB43_19470) 3957184..3957837 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
QCB43_RS19475 (QCB43_19475) 3958102..3958389 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
QCB43_RS19480 (QCB43_19480) 3958382..3958699 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QCB43_RS19485 (QCB43_19485) 3958884..3959927 - 1044 WP_023300815.1 DUF2157 domain-containing protein -
QCB43_RS19490 (QCB43_19490) 3960593..3961459 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
QCB43_RS19495 (QCB43_19495) 3961568..3962995 + 1428 WP_023300814.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T276821 WP_004142563.1 NZ_CP122389:c3958699-3958382 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT276821 WP_004142561.1 NZ_CP122389:c3958389-3958102 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References