Detailed information of TA system TA027682

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 1434109..1434697
• Replicon: chromosome
• Accession: NC_017741
• Organism: Helicobacter pylori Shi112

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: M3P300
• Locus tag: HPSH112_RS06925
• Protein ID: WP_000921941.1
• Coordinates: 1434416..1434697 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: -
• Locus tag: HPSH112_RS06920
• Protein ID: WP_000065283.1
• Coordinates: 1434109..1434423 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPSH112_RS06900	1429670..1430326	+	657	WP_000587367.1	ParA family protein	-
HPSH112_RS06905	1430411..1430680	+	270	WP_000394629.1	hypothetical protein	-
HPSH112_RS06910	1430712..1431896	+	1185	WP_000665505.1	hypothetical protein	-
HPSH112_RS06915	1431921..1433828	-	1908	WP_001162713.1	relaxase/mobilization nuclease domain-containing protein	-
HPSH112_RS06920	1434109..1434423	+	315	WP_000065283.1	hypothetical protein	Antitoxin
HPSH112_RS06925	1434416..1434697	+	282	WP_000921941.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
HPSH112_RS08645	1434972..1436700	-	1729	Protein_1325	type IV secretory system conjugative DNA transfer family protein	-
HPSH112_RS06935	1436701..1437258	-	558	WP_001167785.1	hypothetical protein	-
HPSH112_RS06940	1437259..1437549	-	291	WP_000159821.1	hypothetical protein	-
HPSH112_RS06945	1437546..1438001	-	456	WP_000795438.1	hypothetical protein	-
HPSH112_RS06950	1437998..1438939	-	942	WP_000625127.1	Flp pilus assembly complex ATPase component TadA	-
HPSH112_RS06955	1438939..1439238	-	300	WP_000660134.1	hypothetical protein	-
HPSH112_RS06960	1439235..1439498	-	264	WP_000564645.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	pflA / babA/hopS / rfaJ / fliQ / fliI	1256265..1474035	217770

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10891.80 Da
• Isoelectric Point: 9.5726

>T27682 WP_000921941.1 NC_017741:1434416-1434697 [Helicobacter pylori Shi112]
MLKVRTKKDFLKDFNKHILSGRITESDVTSVVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T27682 NC_017741:1434416-1434697 [Helicobacter pylori Shi112]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCCGGGCGTATTACAGAAAGCGA
TGTTACAAGCGTTGTTGATTGCCTCAAAGAGCAAAAACCACTCCAACAAAAGTATTGCGACCATGCCTTGAGCGGCAATC
TTAAAGGACTGAGAGAATGCCATGTCAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12153.71 Da
• Isoelectric Point: 6.4659

>AT27682 WP_000065283.1 NC_017741:1434109-1434423 [Helicobacter pylori Shi112]
MSNIITDYSQYNEKQLHNFLNAIEKQLLKAEGDKKKAIKKIQECELQEQMIRQVLAQKHSQEKEPTQSLLNTIASKDNPE
CDVSFGDFNDFLQIAKQERERRNA

Nucleotide

• Length: 315 bp

>AT27682 NC_017741:1434109-1434423 [Helicobacter pylori Shi112]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAAAACAACTGCACAATTTCTTAAACGCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAAAAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTAAATACGATCGCCTCAAAAGATAACCCAGAA
TGCGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCGTAATGCTTAA

Structures

Toxin

• AlphaFold DB: M3P300