Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 318904..319625 Replicon plasmid pPAG02
Accession NZ_CP122321
Organism Pantoea agglomerans pv. gypsophilae strain 824-1

Toxin (Protein)


Gene name higB Uniprot ID A0A8X8J2A8
Locus tag A7P62_RS20840 Protein ID WP_010247063.1
Coordinates 319314..319625 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag A7P62_RS20835 Protein ID WP_064690799.1
Coordinates 318904..319317 (-) Length 138 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7P62_RS20805 (A7P62_20805) 314060..314422 - 363 WP_071941147.1 DUF2778 domain-containing protein -
A7P62_RS20810 (A7P62_20810) 314422..314910 - 489 WP_064690961.1 type VI secretion system tube protein TssD -
A7P62_RS20815 (A7P62_20815) 315294..316028 - 735 WP_010247068.1 GntR family transcriptional regulator -
A7P62_RS20820 (A7P62_20820) 316605..317054 + 450 WP_064690797.1 PTS sugar transporter subunit IIA -
A7P62_RS20825 (A7P62_20825) 317103..317375 + 273 WP_010247066.1 PTS sugar transporter subunit IIB -
A7P62_RS20830 (A7P62_20830) 317415..318788 + 1374 WP_064690798.1 PTS ascorbate transporter subunit IIC -
A7P62_RS20835 (A7P62_20835) 318904..319317 - 414 WP_064690799.1 helix-turn-helix domain-containing protein Antitoxin
A7P62_RS20840 (A7P62_20840) 319314..319625 - 312 WP_010247063.1 type II toxin-antitoxin system HigB family toxin Toxin
A7P62_RS20845 (A7P62_20845) 320066..321619 + 1554 WP_064690800.1 aspartate aminotransferase family protein -
A7P62_RS20850 (A7P62_20850) 321630..322922 + 1293 WP_064690801.1 lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hcp/tssD 1..582658 582658


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Antitoxin HigA

(2-137)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12207.12 Da        Isoelectric Point: 9.8340

>T276793 WP_010247063.1 NZ_CP122321:c319625-319314 [Pantoea agglomerans pv. gypsophilae]
MHIISRGPFREAAIIYPNHASALDAAYLVLRDENFATPDALKIRFQSLDRMKYREKWWVIDVGGNSLRIMFYADFDRGKI
FIKHIVTHAEYDKLVKKYRESKE

Download         Length: 312 bp

Antitoxin


Download         Length: 138 a.a.        Molecular weight: 15383.37 Da        Isoelectric Point: 4.9732

>AT276793 WP_064690799.1 NZ_CP122321:c319317-318904 [Pantoea agglomerans pv. gypsophilae]
MITEAIQATNDLVRIVPFLGGSTDKRDYEQALELVEYLVEHQPDSPLVEILSDKVARYENSAPEFAAFNARTDAIPRGVA
LLRVIMDQHGLTQSSFADEIGQHSYVSRILRGDRPLTDKHKARLAARFNLPFEAFAE

Download         Length: 414 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References