Detailed information of TA system TA027678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 1164495..1165083
• Replicon: chromosome
• Accession: NC_017739
• Organism: Helicobacter pylori Shi417

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: M3P300
• Locus tag: HPSH417_RS05550
• Protein ID: WP_000921941.1
• Coordinates: 1164495..1164776 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: -
• Locus tag: HPSH417_RS05555
• Protein ID: WP_000065282.1
• Coordinates: 1164769..1165083 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPSH417_RS05515	1159694..1159958	+	265	Protein_1053	hypothetical protein	-
HPSH417_RS05520	1159955..1160254	+	300	WP_000660158.1	hypothetical protein	-
HPSH417_RS05525	1160254..1161195	+	942	WP_000625128.1	Flp pilus assembly complex ATPase component TadA	-
HPSH417_RS05530	1161192..1161647	+	456	WP_000795438.1	hypothetical protein	-
HPSH417_RS05535	1161644..1161934	+	291	WP_000159821.1	hypothetical protein	-
HPSH417_RS05540	1161935..1162492	+	558	WP_001167785.1	hypothetical protein	-
HPSH417_RS05545	1162493..1164220	+	1728	WP_001208195.1	type IV secretory system conjugative DNA transfer family protein	-
HPSH417_RS05550	1164495..1164776	-	282	WP_000921941.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
HPSH417_RS05555	1164769..1165083	-	315	WP_000065282.1	hypothetical protein	Antitoxin
HPSH417_RS05560	1165346..1167253	+	1908	WP_001162712.1	relaxase/mobilization nuclease domain-containing protein	-
HPSH417_RS05565	1167278..1168455	-	1178	Protein_1063	hypothetical protein	-
HPSH417_RS05570	1168487..1168756	-	270	WP_000394632.1	hypothetical protein	-
HPSH417_RS05575	1168841..1169497	-	657	WP_000587368.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	flgK / flgM / gluP	1081552..1206611	125059

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10891.80 Da
• Isoelectric Point: 9.5726

>T27678 WP_000921941.1 NC_017739:c1164776-1164495 [Helicobacter pylori Shi417]
MLKVRTKKDFLKDFNKHILSGRITESDVTSVVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T27678 NC_017739:c1164776-1164495 [Helicobacter pylori Shi417]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCCGGGCGTATTACAGAAAGCGA
TGTTACAAGCGTTGTTGATTGCCTCAAAGAGCAAAAACCACTCCAACAAAAGTATTGCGACCATGCCTTGAGCGGTAATC
TTAAAGGGTTGAGAGAATGCCATGTCAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12126.64 Da
• Isoelectric Point: 5.2248

>AT27678 WP_000065282.1 NC_017739:c1165083-1164769 [Helicobacter pylori Shi417]
MSNIITDYSQYNEKQLHNFLNAIEKQLLKAEGDKKKAIKKIQECELQEQMIRQVLAQKHSQEKEPTQSLLNTIASKDNPE
CDVSFGDFNDFLQIAKQERERQDA

Nucleotide

• Length: 315 bp

>AT27678 NC_017739:c1165083-1164769 [Helicobacter pylori Shi417]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAAAACAACTGCACAATTTCTTAAACGCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAAAAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTAAATACGATCGCCTCAAAAGATAACCCAGAA
TGCGATGTTTCGTTTGGAGATTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCAAGATGCTTAA

Structures

Toxin

• AlphaFold DB: M3P300