Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2689539..2690233 Replicon chromosome
Accession NZ_CP122319
Organism Escherichia coli strain BW25113

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag QCG47_RS12850 Protein ID WP_001263489.1
Coordinates 2689835..2690233 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag QCG47_RS12845 Protein ID WP_000554758.1
Coordinates 2689539..2689832 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QCG47_RS12825 (2685171) 2685171..2685668 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
QCG47_RS12830 (2685892) 2685892..2687604 - 1713 Protein_2484 flagellar biosynthesis protein FlhA -
QCG47_RS12835 (2687576) 2687576..2688361 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
QCG47_RS12840 (2688432) 2688432..2689487 + 1056 WP_001226164.1 DNA polymerase IV -
QCG47_RS12845 (2689539) 2689539..2689832 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QCG47_RS12850 (2689835) 2689835..2690233 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QCG47_RS12855 (2690243) 2690243..2690695 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
QCG47_RS12860 (2691013) 2691013..2691219 + 207 Protein_2490 RtcB family protein -
QCG47_RS12865 (2691215) 2691215..2691736 + 522 Protein_2491 peptide chain release factor H -
QCG47_RS12870 (2691793) 2691793..2693250 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
QCG47_RS12875 (2693511) 2693511..2693969 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (2694565) 2694565..2694645 + 81 NuclAT_12 - -
- (2694565) 2694565..2694645 + 81 NuclAT_12 - -
- (2694565) 2694565..2694645 + 81 NuclAT_12 - -
- (2694565) 2694565..2694645 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T276763 WP_001263489.1 NZ_CP122319:2689835-2690233 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT276763 WP_000554758.1 NZ_CP122319:2689539-2689832 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References