Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2254740..2255434 Replicon chromosome
Accession NZ_CP122318
Organism Escherichia coli strain HT873X1

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag QCG45_RS11160 Protein ID WP_001263489.1
Coordinates 2255036..2255434 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag QCG45_RS11155 Protein ID WP_000554758.1
Coordinates 2254740..2255033 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QCG45_RS11135 (2250372) 2250372..2250869 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
QCG45_RS11140 (2251093) 2251093..2252805 - 1713 Protein_2170 flagellar biosynthesis protein FlhA -
QCG45_RS11145 (2252777) 2252777..2253562 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
QCG45_RS11150 (2253633) 2253633..2254688 + 1056 WP_001226164.1 DNA polymerase IV -
QCG45_RS11155 (2254740) 2254740..2255033 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QCG45_RS11160 (2255036) 2255036..2255434 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QCG45_RS11165 (2255444) 2255444..2255896 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
QCG45_RS11170 (2256214) 2256214..2256420 + 207 Protein_2176 RtcB family protein -
QCG45_RS11175 (2256416) 2256416..2256937 + 522 Protein_2177 peptide chain release factor H -
QCG45_RS11180 (2256994) 2256994..2258451 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
QCG45_RS11185 (2258712) 2258712..2259170 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (2259766) 2259766..2259846 + 81 NuclAT_12 - -
- (2259766) 2259766..2259846 + 81 NuclAT_12 - -
- (2259766) 2259766..2259846 + 81 NuclAT_12 - -
- (2259766) 2259766..2259846 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T276733 WP_001263489.1 NZ_CP122318:2255036-2255434 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT276733 WP_000554758.1 NZ_CP122318:2254740-2255033 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References