Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3796577..3797271 Replicon chromosome
Accession NZ_CP122317
Organism Escherichia coli strain HT-948

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag QCG49_RS18410 Protein ID WP_001263489.1
Coordinates 3796577..3796975 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag QCG49_RS18415 Protein ID WP_000554758.1
Coordinates 3796978..3797271 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3792165) 3792165..3792245 - 81 NuclAT_12 - -
- (3792165) 3792165..3792245 - 81 NuclAT_12 - -
- (3792165) 3792165..3792245 - 81 NuclAT_12 - -
- (3792165) 3792165..3792245 - 81 NuclAT_12 - -
QCG49_RS18385 (3792841) 3792841..3793299 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
QCG49_RS18390 (3793560) 3793560..3795017 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
QCG49_RS18395 (3795074) 3795074..3795595 - 522 Protein_3583 peptide chain release factor H -
QCG49_RS18400 (3795591) 3795591..3795797 - 207 Protein_3584 RtcB family protein -
QCG49_RS18405 (3796115) 3796115..3796567 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
QCG49_RS18410 (3796577) 3796577..3796975 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QCG49_RS18415 (3796978) 3796978..3797271 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QCG49_RS18420 (3797323) 3797323..3798378 - 1056 WP_001226164.1 DNA polymerase IV -
QCG49_RS18425 (3798449) 3798449..3799234 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
QCG49_RS18430 (3799206) 3799206..3800918 + 1713 Protein_3590 flagellar biosynthesis protein FlhA -
QCG49_RS18435 (3801142) 3801142..3801639 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T276709 WP_001263489.1 NZ_CP122317:c3796975-3796577 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT276709 WP_000554758.1 NZ_CP122317:c3797271-3796978 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References