Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2221324..2222018 Replicon chromosome
Accession NZ_CP122316
Organism Escherichia coli strain HT-946

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag QCG48_RS11030 Protein ID WP_001263489.1
Coordinates 2221620..2222018 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag QCG48_RS11025 Protein ID WP_000554758.1
Coordinates 2221324..2221617 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QCG48_RS11005 (2216956) 2216956..2217453 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
QCG48_RS11010 (2217677) 2217677..2219389 - 1713 Protein_2144 flagellar biosynthesis protein FlhA -
QCG48_RS11015 (2219361) 2219361..2220146 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
QCG48_RS11020 (2220217) 2220217..2221272 + 1056 WP_001226164.1 DNA polymerase IV -
QCG48_RS11025 (2221324) 2221324..2221617 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QCG48_RS11030 (2221620) 2221620..2222018 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QCG48_RS11035 (2222028) 2222028..2222480 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
QCG48_RS11040 (2222798) 2222798..2223004 + 207 Protein_2150 RtcB family protein -
QCG48_RS11045 (2223000) 2223000..2223521 + 522 Protein_2151 peptide chain release factor H -
QCG48_RS11050 (2223578) 2223578..2225035 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
QCG48_RS11055 (2225296) 2225296..2225754 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (2226350) 2226350..2226430 + 81 NuclAT_12 - -
- (2226350) 2226350..2226430 + 81 NuclAT_12 - -
- (2226350) 2226350..2226430 + 81 NuclAT_12 - -
- (2226350) 2226350..2226430 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T276671 WP_001263489.1 NZ_CP122316:2221620-2222018 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT276671 WP_000554758.1 NZ_CP122316:2221324-2221617 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References