276634

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2070731..2071425 Replicon chromosome
Accession NZ_CP122315
Organism Escherichia coli strain HT-947

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag QCG46_RS10050 Protein ID WP_001263489.1
Coordinates 2070731..2071129 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag QCG46_RS10055 Protein ID WP_000554758.1
Coordinates 2071132..2071425 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (2066319) 2066319..2066399 - 81 NuclAT_12 - -
- (2066319) 2066319..2066399 - 81 NuclAT_12 - -
- (2066319) 2066319..2066399 - 81 NuclAT_12 - -
- (2066319) 2066319..2066399 - 81 NuclAT_12 - -
QCG46_RS10025 (2066995) 2066995..2067453 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
QCG46_RS10030 (2067714) 2067714..2069171 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
QCG46_RS10035 (2069228) 2069228..2069749 - 522 Protein_1948 peptide chain release factor H -
QCG46_RS10040 (2069745) 2069745..2069951 - 207 Protein_1949 RtcB family protein -
QCG46_RS10045 (2070269) 2070269..2070721 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
QCG46_RS10050 (2070731) 2070731..2071129 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
QCG46_RS10055 (2071132) 2071132..2071425 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
QCG46_RS10060 (2071477) 2071477..2072532 - 1056 WP_001226164.1 DNA polymerase IV -
QCG46_RS10065 (2072603) 2072603..2073388 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
QCG46_RS10070 (2073360) 2073360..2075072 + 1713 Protein_1955 flagellar biosynthesis protein FlhA -
QCG46_RS10075 (2075296) 2075296..2075793 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T276634 WP_001263489.1 NZ_CP122315:c2071129-2070731 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT276634 WP_000554758.1 NZ_CP122315:c2071425-2071132 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References