Detailed information of TA system TA027657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4300285..4300887
• Replicon: chromosome
• Accession: NC_017664
• Organism: Escherichia coli W

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: WFL_RS21440
• Protein ID: WP_000897305.1
• Coordinates: 4300285..4300596 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: WFL_RS21445
• Protein ID: WP_000356397.1
• Coordinates: 4300597..4300887 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WFL_RS21410	4295315..4296100	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
WFL_RS21415	4296199..4296798	+	600	WP_001315111.1	glucose-1-phosphatase	-
WFL_RS21420	4296792..4297664	+	873	WP_000920762.1	virulence factor BrkB family protein	-
WFL_RS21425	4297661..4298098	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
WFL_RS21430	4298143..4299084	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
WFL_RS21435	4299148..4300056	-	909	WP_001162704.1	alpha/beta hydrolase	-
WFL_RS21440	4300285..4300596	+	312	WP_000897305.1	hypothetical protein	Toxin
WFL_RS21445	4300597..4300887	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
WFL_RS21450	4301492..4301710	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
WFL_RS21455	4301929..4302171	+	243	WP_001086388.1	hypothetical protein	-
WFL_RS21465	4302501..4303430	-	930	WP_000027702.1	formate dehydrogenase accessory protein FdhE	-
WFL_RS21470	4303427..4304062	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
WFL_RS21475	4304059..4304961	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T27657 WP_000897305.1 NC_017664:4300285-4300596 [Escherichia coli W]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T27657 NC_017664:4300285-4300596 [Escherichia coli W]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT27657 WP_000356397.1 NC_017664:4300597-4300887 [Escherichia coli W]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT27657 NC_017664:4300597-4300887 [Escherichia coli W]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7