Detailed information of TA system TA027654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3491290..3492089
• Replicon: chromosome
• Accession: NC_017664
• Organism: Escherichia coli W

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: U9XVR9
• Locus tag: WFL_RS17410
• Protein ID: WP_000347267.1
• Coordinates: 3491625..3492089 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: WFL_RS17405
• Protein ID: WP_001307405.1
• Coordinates: 3491290..3491625 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WFL_RS17385	3487075..3487845	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
WFL_RS17390	3487861..3489195	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
WFL_RS17400	3489570..3491141	+	1572	WP_001273741.1	galactarate dehydratase	-
WFL_RS17405	3491290..3491625	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
WFL_RS17410	3491625..3492089	+	465	WP_000347267.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
WFL_RS17415	3492144..3492953	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
WFL_RS17420	3493202..3494482	+	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
WFL_RS17425	3494505..3494978	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
WFL_RS17430	3494989..3495768	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
WFL_RS17435	3495758..3496636	+	879	WP_001298758.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
WFL_RS17440	3496654..3497088	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3480415..3492089	11674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17807.17 Da
• Isoelectric Point: 9.4947

>T27654 WP_000347267.1 NC_017664:3491625-3492089 [Escherichia coli W]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T27654 NC_017664:3491625-3492089 [Escherichia coli W]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT27654 WP_001307405.1 NC_017664:3491290-3491625 [Escherichia coli W]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT27654 NC_017664:3491290-3491625 [Escherichia coli W]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACACGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0XTR4

Antitoxin

• AlphaFold DB: A0A0E0XVC7