27650

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2224394..2224896 Replicon chromosome
Accession NC_017664
Organism Escherichia coli W

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag WFL_RS11175 Protein ID WP_000767829.1
Coordinates 2224394..2224648 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag WFL_RS11180 Protein ID WP_001259255.1
Coordinates 2224645..2224896 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WFL_RS11150 2219409..2219636 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
WFL_RS11155 2219650..2220708 - 1059 WP_000492346.1 transport protein YeeE -
WFL_RS11160 2220887..2222245 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
WFL_RS27435 2222235..2222297 - 63 WP_010723108.1 membrane protein YoeI -
WFL_RS11165 2222512..2223441 - 930 WP_000803351.1 LysR family transcriptional regulator -
WFL_RS11170 2223487..2224311 - 825 WP_000754749.1 SDR family oxidoreductase -
WFL_RS11175 2224394..2224648 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
WFL_RS11180 2224645..2224896 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
WFL_RS25860 2225179..2225229 + 51 WP_001364200.1 his operon leader peptide -
WFL_RS11185 2225375..2226274 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
WFL_RS11190 2226280..2227584 + 1305 WP_001349967.1 histidinol dehydrogenase -
WFL_RS11195 2227581..2228651 + 1071 WP_000108957.1 histidinol-phosphate transaminase -
WFL_RS11200 2228651..2229718 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T27650 WP_000767829.1 NC_017664:c2224648-2224394 [Escherichia coli W]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T27650 NC_017664:c2224648-2224394 [Escherichia coli W]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT27650 WP_001259255.1 NC_017664:c2224896-2224645 [Escherichia coli W]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT27650 NC_017664:c2224896-2224645 [Escherichia coli W]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References