Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2224394..2224896 Replicon chromosome
Accession NC_017664
Organism Escherichia coli W

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag WFL_RS11175 Protein ID WP_000767829.1
Coordinates 2224394..2224648 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag WFL_RS11180 Protein ID WP_001259255.1
Coordinates 2224645..2224896 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WFL_RS11150 2219409..2219636 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
WFL_RS11155 2219650..2220708 - 1059 WP_000492346.1 transport protein YeeE -
WFL_RS11160 2220887..2222245 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
WFL_RS27435 2222235..2222297 - 63 WP_010723108.1 membrane protein YoeI -
WFL_RS11165 2222512..2223441 - 930 WP_000803351.1 LysR family transcriptional regulator -
WFL_RS11170 2223487..2224311 - 825 WP_000754749.1 SDR family oxidoreductase -
WFL_RS11175 2224394..2224648 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
WFL_RS11180 2224645..2224896 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
WFL_RS25860 2225179..2225229 + 51 WP_001364200.1 his operon leader peptide -
WFL_RS11185 2225375..2226274 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
WFL_RS11190 2226280..2227584 + 1305 WP_001349967.1 histidinol dehydrogenase -
WFL_RS11195 2227581..2228651 + 1071 WP_000108957.1 histidinol-phosphate transaminase -
WFL_RS11200 2228651..2229718 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T27650 WP_000767829.1 NC_017664:c2224648-2224394 [Escherichia coli W]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T27650 NC_017664:c2224648-2224394 [Escherichia coli W]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT27650 WP_001259255.1 NC_017664:c2224896-2224645 [Escherichia coli W]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT27650 NC_017664:c2224896-2224645 [Escherichia coli W]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References