Detailed information of TA system TA027635

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1498903..1499274
• Replicon: chromosome
• Accession: NC_017664
• Organism: Escherichia coli W

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: WFL_RS07420
• Protein ID: WP_001317028.1
• Coordinates: 1499080..1499274 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1498903..1499081 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WFL_RS07395	1494858..1496231	+	1374	WP_000123740.1	ATP-dependent RNA helicase DbpA	-
WFL_RS07400	1496360..1497295	-	936	WP_001157422.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
WFL_RS25620	1497347..1498582	-	1236	WP_000040852.1	site-specific integrase	-
WFL_RS07410	1498584..1498799	-	216	WP_000079604.1	excisionase XisR	-
WFL_RS07415	1498878..1499087	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	1498903..1499081	+	179	NuclAT_0	-	Antitoxin
-	1498903..1499081	+	179	NuclAT_0	-	Antitoxin
-	1498903..1499081	+	179	NuclAT_0	-	Antitoxin
-	1498903..1499081	+	179	NuclAT_0	-	Antitoxin
WFL_RS07420	1499080..1499274	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
WFL_RS07425	1499331..1500140	-	810	WP_000166319.1	recombination protein RecT	-
WFL_RS07430	1500133..1502733	-	2601	WP_000105152.1	exodeoxyribonuclease VIII	-
WFL_RS07435	1502835..1503110	-	276	WP_001349884.1	hypothetical protein	-
WFL_RS07440	1503185..1503355	-	171	WP_001349883.1	hypothetical protein	-
WFL_RS07445	1503355..1503576	-	222	WP_000560220.1	killing protein KilR	-
WFL_RS25070	1503997..1504149	-	153	WP_001169153.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1497347..1546203	48856

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T27635 WP_001317028.1 NC_017664:c1499274-1499080 [Escherichia coli W]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T27635 NC_017664:c1499274-1499080 [Escherichia coli W]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGGTATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT27635 NC_017664:1498903-1499081 [Escherichia coli W]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAATTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin