Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4234591..4235132 Replicon chromosome
Accession NZ_CP121356
Organism Escherichia coli strain rl0044

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag P9060_RS21450 Protein ID WP_000615976.1
Coordinates 4234854..4235132 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag P9060_RS21445 Protein ID WP_000729704.1
Coordinates 4234591..4234851 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P9060_RS21425 (4230266) 4230266..4231051 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
P9060_RS21430 (4231023) 4231023..4232735 + 1713 Protein_4201 flagellar biosynthesis protein FlhA -
P9060_RS21435 (4232959) 4232959..4233456 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
P9060_RS21440 (4233632) 4233632..4234405 - 774 WP_015675129.1 C40 family peptidase -
P9060_RS21445 (4234591) 4234591..4234851 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
P9060_RS21450 (4234854) 4234854..4235132 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
P9060_RS21455 (4235288) 4235288..4236028 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
P9060_RS21460 (4235999) 4235999..4236766 - 768 WP_000333379.1 class II glutamine amidotransferase -
P9060_RS21465 (4236972) 4236972..4237550 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T276311 WP_000615976.1 NZ_CP121356:4234854-4235132 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT276311 WP_000729704.1 NZ_CP121356:4234591-4234851 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References