276310

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4228394..4229088 Replicon chromosome
Accession NZ_CP121356
Organism Escherichia coli strain rl0044

Toxin (Protein)


Gene name yafO Uniprot ID A0A3A6T3I6
Locus tag P9060_RS21410 Protein ID WP_001263490.1
Coordinates 4228394..4228792 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag P9060_RS21415 Protein ID WP_000554757.1
Coordinates 4228795..4229088 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (4224054) 4224054..4224134 - 81 NuclAT_9 - -
- (4224054) 4224054..4224134 - 81 NuclAT_9 - -
- (4224054) 4224054..4224134 - 81 NuclAT_9 - -
- (4224054) 4224054..4224134 - 81 NuclAT_9 - -
P9060_RS21380 (4223394) 4223394..4224638 - 1245 WP_000189541.1 esterase FrsA -
P9060_RS21385 (4224730) 4224730..4225188 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
P9060_RS21390 (4225449) 4225449..4226906 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
P9060_RS21395 (4226963) 4226963..4227484 - 522 Protein_4194 peptide chain release factor H -
P9060_RS21400 (4227483) 4227483..4227686 - 204 Protein_4195 RtcB family protein -
P9060_RS21405 (4227932) 4227932..4228384 - 453 WP_001059847.1 GNAT family N-acetyltransferase -
P9060_RS21410 (4228394) 4228394..4228792 - 399 WP_001263490.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
P9060_RS21415 (4228795) 4228795..4229088 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
P9060_RS21420 (4229140) 4229140..4230195 - 1056 WP_001226164.1 DNA polymerase IV -
P9060_RS21425 (4230266) 4230266..4231051 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
P9060_RS21430 (4231023) 4231023..4232735 + 1713 Protein_4201 flagellar biosynthesis protein FlhA -
P9060_RS21435 (4232959) 4232959..4233456 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4205387..4234258 28871


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15486.86 Da        Isoelectric Point: 8.0949

>T276310 WP_001263490.1 NZ_CP121356:c4228792-4228394 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLLYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp


Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT276310 WP_000554757.1 NZ_CP121356:c4229088-4228795 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A3A6T3I6


Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References