Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4658057..4658598 Replicon chromosome
Accession NZ_CP121294
Organism Escherichia coli O155:H21 strain NWU_1

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag P9N54_RS22575 Protein ID WP_000615976.1
Coordinates 4658320..4658598 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag P9N54_RS22570 Protein ID WP_000729704.1
Coordinates 4658057..4658317 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P9N54_RS22545 (4653840) 4653840..4654625 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
P9N54_RS22550 (4654597) 4654597..4656309 + 1713 Protein_4441 flagellar biosynthesis protein FlhA -
P9N54_RS22555 (4656414) 4656414..4656692 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
P9N54_RS22560 (4656685) 4656685..4657041 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
P9N54_RS22565 (4657098) 4657098..4657871 - 774 WP_000543895.1 C40 family peptidase -
P9N54_RS22570 (4658057) 4658057..4658317 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
P9N54_RS22575 (4658320) 4658320..4658598 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
P9N54_RS22580 (4658754) 4658754..4659494 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
P9N54_RS22585 (4659465) 4659465..4660232 - 768 WP_000333380.1 class II glutamine amidotransferase -
P9N54_RS22590 (4660438) 4660438..4661016 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T276210 WP_000615976.1 NZ_CP121294:4658320-4658598 [Escherichia coli O155:H21]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT276210 WP_000729704.1 NZ_CP121294:4658057-4658317 [Escherichia coli O155:H21]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References