Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2727860..2728362 Replicon chromosome
Accession NC_017660
Organism Escherichia coli KO11FL

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag KO11_RS13335 Protein ID WP_000767829.1
Coordinates 2728108..2728362 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag KO11_RS13330 Protein ID WP_001259255.1
Coordinates 2727860..2728111 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KO11_RS13310 2723038..2724105 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
KO11_RS13315 2724105..2725175 - 1071 WP_000108957.1 histidinol-phosphate transaminase -
KO11_RS13320 2725172..2726476 - 1305 WP_001349967.1 histidinol dehydrogenase -
KO11_RS13325 2726482..2727381 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
KO11_RS25840 2727527..2727577 - 51 WP_001364200.1 his operon leader peptide -
KO11_RS13330 2727860..2728111 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
KO11_RS13335 2728108..2728362 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
KO11_RS13340 2728445..2729269 + 825 WP_000754749.1 SDR family oxidoreductase -
KO11_RS13345 2729315..2730244 + 930 WP_000803351.1 LysR family transcriptional regulator -
KO11_RS27765 2730459..2730521 + 63 WP_010723108.1 membrane protein YoeI -
KO11_RS13350 2730511..2731869 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
KO11_RS13355 2732048..2733106 + 1059 WP_000492346.1 transport protein YeeE -
KO11_RS13360 2733120..2733347 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T27615 WP_000767829.1 NC_017660:2728108-2728362 [Escherichia coli KO11FL]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T27615 NC_017660:2728108-2728362 [Escherichia coli KO11FL]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT27615 WP_001259255.1 NC_017660:2727860-2728111 [Escherichia coli KO11FL]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT27615 NC_017660:2727860-2728111 [Escherichia coli KO11FL]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References