Detailed information of TA system TA276099

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3822408..3822633
• Replicon: chromosome
• Accession: NZ_CP121223
• Organism: Shigella flexneri 2a strain Sflex 21-42

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: P5F89_RS21015
• Protein ID: WP_000813254.1
• Coordinates: 3822478..3822633 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3822408..3822466 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P5F89_RS20975	3818406..3818575	+	170	Protein_3676	hypothetical protein	-
P5F89_RS20980	3818721..3819467	+	747	WP_000788996.1	ATP-binding protein	-
P5F89_RS20985	3819482..3819904	+	423	WP_001118168.1	DUF977 family protein	-
P5F89_RS20990	3819962..3820318	+	357	WP_005048249.1	hypothetical protein	-
P5F89_RS20995	3820411..3820629	+	219	WP_000256998.1	DUF4014 family protein	-
P5F89_RS21000	3820631..3820996	+	366	WP_001229297.1	HNH endonuclease signature motif containing protein	-
P5F89_RS21005	3820993..3821658	+	666	WP_000208062.1	hypothetical protein	-
P5F89_RS21010	3821658..3822023	+	366	WP_000610655.1	DUF551 domain-containing protein	-
-	3822408..3822466	-	59	-	-	Antitoxin
P5F89_RS21015	3822478..3822633	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
P5F89_RS21025	3823970..3824569	+	600	WP_094107379.1	DUF1367 family protein	-
P5F89_RS21030	3824569..3824859	+	291	WP_000228038.1	DUF1364 domain-containing protein	-
P5F89_RS21035	3824856..3825410	+	555	WP_289228590.1	DUF1133 family protein	-
P5F89_RS21040	3825563..3825736	+	174	WP_000504450.1	hypothetical protein	-
P5F89_RS21045	3825798..3826937	+	1140	WP_000088354.1	IS3 family transposase	-
P5F89_RS21050	3826930..3827490	+	561	WP_072075205.1	ORF6N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	3810395..3876367	65972

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T276099 WP_000813254.1 NZ_CP121223:3822478-3822633 [Shigella flexneri 2a]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

Antitoxin

Nucleotide

• Length: 59 bp

>AT276099 NZ_CP121223:c3822466-3822408 [Shigella flexneri 2a]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin