Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2687814..2688508 Replicon chromosome
Accession NZ_CP121223
Organism Shigella flexneri 2a strain Sflex 21-42

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag P5F89_RS15180 Protein ID WP_001263491.1
Coordinates 2688110..2688508 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag P5F89_RS15175 Protein ID WP_000554759.1
Coordinates 2687814..2688107 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5F89_RS15155 (2683453) 2683453..2683950 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
P5F89_RS15160 (2684167) 2684167..2685879 - 1713 Protein_2541 flagellar biosynthesis protein FlhA -
P5F89_RS15165 (2685851) 2685851..2686636 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
P5F89_RS15170 (2686707) 2686707..2687762 + 1056 WP_001226172.1 DNA polymerase IV -
P5F89_RS15175 (2687814) 2687814..2688107 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
P5F89_RS15180 (2688110) 2688110..2688508 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
P5F89_RS15185 (2688518) 2688518..2688970 + 453 WP_001059860.1 GNAT family N-acetyltransferase -
P5F89_RS15190 (2689216) 2689216..2690259 + 1044 WP_005053205.1 RNA ligase RtcB family protein -
P5F89_RS15195 (2690375) 2690375..2690935 + 561 Protein_2548 peptide chain release factor H -
P5F89_RS15200 (2690992) 2690992..2692449 - 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
P5F89_RS15205 (2692711) 2692711..2693169 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T276095 WP_001263491.1 NZ_CP121223:2688110-2688508 [Shigella flexneri 2a]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT276095 WP_000554759.1 NZ_CP121223:2687814-2688107 [Shigella flexneri 2a]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References