Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 996157..996884 Replicon chromosome
Accession NZ_CP121223
Organism Shigella flexneri 2a strain Sflex 21-42

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag P5F89_RS06885 Protein ID WP_000550189.1
Coordinates 996570..996884 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag P5F89_RS06880 Protein ID WP_000560266.1
Coordinates 996157..996573 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5F89_RS06870 (991517) 991517..993868 + 2352 WP_000695506.1 alpha-glucosidase -
P5F89_RS06875 (994094) 994094..996112 + 2019 WP_000121487.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
P5F89_RS06880 (996157) 996157..996573 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
P5F89_RS06885 (996570) 996570..996884 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
P5F89_RS06890 (997169) 997169..998305 - 1137 WP_000018656.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
P5F89_RS06895 (998390) 998390..998893 + 504 WP_005050988.1 M48 family metallopeptidase -
P5F89_RS06900 (998970) 998970..999662 + 693 WP_000942538.1 vancomycin high temperature exclusion protein -
P5F89_RS06905 (999741) 999741..1000739 + 999 WP_005050984.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T276093 WP_000550189.1 NZ_CP121223:c996884-996570 [Shigella flexneri 2a]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT276093 WP_000560266.1 NZ_CP121223:c996573-996157 [Shigella flexneri 2a]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References