Detailed information of TA system TA027608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 674335..674937
• Replicon: chromosome
• Accession: NC_017660
• Organism: Escherichia coli KO11FL

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KO11_RS03310
• Protein ID: WP_000897305.1
• Coordinates: 674626..674937 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KO11_RS03305
• Protein ID: WP_000356397.1
• Coordinates: 674335..674625 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KO11_RS03275	670261..671163	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KO11_RS03280	671160..671795	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KO11_RS03285	671792..672721	+	930	WP_000027702.1	formate dehydrogenase accessory protein FdhE	-
KO11_RS03295	673051..673293	-	243	WP_001086388.1	hypothetical protein	-
KO11_RS03300	673512..673730	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
KO11_RS03305	674335..674625	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
KO11_RS03310	674626..674937	-	312	WP_000897305.1	hypothetical protein	Toxin
KO11_RS03315	675166..676074	+	909	WP_001162704.1	alpha/beta hydrolase	-
KO11_RS03320	676138..677079	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KO11_RS03325	677124..677561	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
KO11_RS03330	677558..678430	-	873	WP_000920762.1	virulence factor BrkB family protein	-
KO11_RS03335	678424..679023	-	600	WP_001315111.1	glucose-1-phosphatase	-
KO11_RS03340	679122..679907	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T27608 WP_000897305.1 NC_017660:c674937-674626 [Escherichia coli KO11FL]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T27608 NC_017660:c674937-674626 [Escherichia coli KO11FL]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT27608 WP_000356397.1 NC_017660:c674625-674335 [Escherichia coli KO11FL]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT27608 NC_017660:c674625-674335 [Escherichia coli KO11FL]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7