Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2393730..2394292 Replicon chromosome
Accession NZ_CP121199
Organism Escherichia coli strain E50

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag P6H83_RS11490 Protein ID WP_000605676.1
Coordinates 2394014..2394292 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag P6H83_RS11485 Protein ID WP_000781370.1
Coordinates 2393730..2394014 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6H83_RS11470 (2389089) 2389089..2392136 + 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -
P6H83_RS11475 (2392149) 2392149..2393033 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
P6H83_RS11480 (2393026) 2393026..2393679 + 654 WP_000045650.1 formate dehydrogenase-N subunit gamma -
P6H83_RS11485 (2393730) 2393730..2394014 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
P6H83_RS11490 (2394014) 2394014..2394292 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
P6H83_RS11495 (2394478) 2394478..2395488 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
P6H83_RS11500 (2395622) 2395622..2397319 - 1698 WP_000433464.1 malate dehydrogenase -
P6H83_RS11505 (2397476) 2397476..2397613 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
P6H83_RS11510 (2397715) 2397715..2397930 - 216 WP_000495766.1 biofilm-dependent modulation protein -
P6H83_RS11515 (2398275) 2398275..2398706 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T276042 WP_000605676.1 NZ_CP121199:c2394292-2394014 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT276042 WP_000781370.1 NZ_CP121199:c2394014-2393730 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References