Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 653626..654425 Replicon chromosome
Accession NZ_CP121199
Organism Escherichia coli strain E50

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag P6H83_RS03150 Protein ID WP_000347273.1
Coordinates 653626..654090 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID D6JF08
Locus tag P6H83_RS03155 Protein ID WP_001308975.1
Coordinates 654090..654425 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6H83_RS03120 (648627) 648627..649061 - 435 WP_000948834.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
P6H83_RS03125 (649079) 649079..649957 - 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
P6H83_RS03130 (649947) 649947..650726 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
P6H83_RS03135 (650737) 650737..651210 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
P6H83_RS03140 (651233) 651233..652513 - 1281 WP_000681961.1 tagatose-bisphosphate aldolase subunit KbaZ -
P6H83_RS03145 (652762) 652762..653571 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
P6H83_RS03150 (653626) 653626..654090 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
P6H83_RS03155 (654090) 654090..654425 - 336 WP_001308975.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
P6H83_RS03160 (654574) 654574..656145 - 1572 WP_001273756.1 galactarate dehydratase -
P6H83_RS03165 (656520) 656520..657854 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
P6H83_RS03170 (657870) 657870..658640 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T276036 WP_000347273.1 NZ_CP121199:c654090-653626 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12343.97 Da        Isoelectric Point: 4.8616

>AT276036 WP_001308975.1 NZ_CP121199:c654425-654090 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB D6JF08

References