Detailed information of TA system TA027602

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 104011..104437
• Replicon: plasmid pO83_CORR
• Accession: NC_017659
• Organism: Escherichia coli O83:H1 str. NRG 857C

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: NRG857_RS23320
• Protein ID: WP_001312861.1
• Coordinates: 104011..104169 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	104213..104437 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NRG857_RS23290	99386..100075	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
NRG857_RS23295	100262..100645	-	384	WP_001151524.1	relaxosome protein TraM	-
NRG857_RS23300	100966..101568	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
NRG857_RS23305	101864..102685	-	822	WP_001234426.1	DUF945 domain-containing protein	-
NRG857_RS23310	102803..103090	-	288	WP_000107535.1	hypothetical protein	-
NRG857_RS23320	104011..104169	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	104213..104437	-	225	NuclAT_0	-	Antitoxin
-	104213..104437	-	225	NuclAT_0	-	Antitoxin
-	104213..104437	-	225	NuclAT_0	-	Antitoxin
-	104213..104437	-	225	NuclAT_0	-	Antitoxin
NRG857_RS23325	104249..104437	+	189	WP_001299721.1	hypothetical protein	-
NRG857_RS23330	104449..105168	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
NRG857_RS23335	105165..105599	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
NRG857_RS23340	105654..105851	-	198	Protein_114	hypothetical protein	-
NRG857_RS23345	105879..106112	-	234	WP_000005990.1	DUF905 family protein	-
NRG857_RS23350	106185..106724	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
NRG857_RS23620	106750..106956	-	207	WP_000275856.1	hypothetical protein	-
NRG857_RS23365	108052..109023	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / catA1 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA1 / ant(3'')-Ia / qacE / sul1 / mph(B) / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..147060	147060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T27602 WP_001312861.1 NC_017659:c104169-104011 [Escherichia coli O83:H1 str. NRG 857C]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T27602 NC_017659:c104169-104011 [Escherichia coli O83:H1 str. NRG 857C]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT27602 NC_017659:c104437-104213 [Escherichia coli O83:H1 str. NRG 857C]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin