Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3616158..3616837 Replicon chromosome
Accession NZ_CP121155
Organism Escherichia coli strain E67

Toxin (Protein)


Gene name higB Uniprot ID A0A0A1A1R1
Locus tag P6H85_RS17525 Protein ID WP_000057541.1
Coordinates 3616535..3616837 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag P6H85_RS17520 Protein ID WP_000806442.1
Coordinates 3616158..3616499 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6H85_RS17510 (3612401) 3612401..3613333 - 933 WP_000883039.1 glutaminase A -
P6H85_RS17515 (3613596) 3613596..3616100 + 2505 WP_061092784.1 copper-exporting P-type ATPase CopA -
P6H85_RS17520 (3616158) 3616158..3616499 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
P6H85_RS17525 (3616535) 3616535..3616837 - 303 WP_000057541.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
P6H85_RS17530 (3616970) 3616970..3617764 + 795 WP_000365157.1 TraB/GumN family protein -
P6H85_RS17535 (3617968) 3617968..3618447 + 480 WP_000186626.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
P6H85_RS17540 (3618484) 3618484..3620136 - 1653 WP_000771723.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
P6H85_RS17545 (3620354) 3620354..3621574 + 1221 WP_001251634.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11795.37 Da        Isoelectric Point: 10.2638

>T275964 WP_000057541.1 NZ_CP121155:c3616837-3616535 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT275964 WP_000806442.1 NZ_CP121155:c3616499-3616158 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A1A1R1

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References