27592

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4964139..4964636 Replicon chromosome
Accession NC_017656
Organism Escherichia coli O55:H7 str. RM12579

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECO55CA74_RS24890 Protein ID WP_001173343.1
Coordinates 4964463..4964636 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ECO55CA74_RS24885 Protein ID WP_000398619.1
Coordinates 4964139..4964435 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO55CA74_RS24865 4960870..4961589 - 720 WP_000611288.1 two-component system response regulator DcuR -
ECO55CA74_RS24870 4961586..4963217 - 1632 WP_001216477.1 sensor histidine kinase -
ECO55CA74_RS24875 4963398..4963628 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
ECO55CA74_RS24880 4963640..4963912 + 273 WP_000405642.1 N-acetyltransferase -
ECO55CA74_RS24885 4964139..4964435 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECO55CA74_RS24890 4964463..4964636 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECO55CA74_RS24895 4964755..4966272 - 1518 WP_001295074.1 lysine--tRNA ligase -
ECO55CA74_RS24900 4966509..4967966 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4964139..4973490 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T27592 WP_001173343.1 NC_017656:4964463-4964636 [Escherichia coli O55:H7 str. RM12579]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T27592 NC_017656:4964463-4964636 [Escherichia coli O55:H7 str. RM12579]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT27592 WP_000398619.1 NC_017656:4964139-4964435 [Escherichia coli O55:H7 str. RM12579]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT27592 NC_017656:4964139-4964435 [Escherichia coli O55:H7 str. RM12579]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCAGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References