Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4197089..4197630 Replicon chromosome
Accession NZ_CP121151
Organism Escherichia coli strain E47

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag P6H31_RS20905 Protein ID WP_000615976.1
Coordinates 4197352..4197630 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag P6H31_RS20900 Protein ID WP_000729704.1
Coordinates 4197089..4197349 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6H31_RS20875 (4192872) 4192872..4193657 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
P6H31_RS20880 (4193629) 4193629..4195341 + 1713 Protein_4098 flagellar biosynthesis protein FlhA -
P6H31_RS20885 (4195446) 4195446..4195724 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
P6H31_RS20890 (4195717) 4195717..4196073 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
P6H31_RS20895 (4196130) 4196130..4196903 - 774 WP_122996642.1 C40 family peptidase -
P6H31_RS20900 (4197089) 4197089..4197349 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
P6H31_RS20905 (4197352) 4197352..4197630 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
P6H31_RS20910 (4197786) 4197786..4198526 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
P6H31_RS20915 (4198497) 4198497..4199264 - 768 WP_000333380.1 class II glutamine amidotransferase -
P6H31_RS20920 (4199470) 4199470..4200048 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR 4195717..4213616 17899


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T275915 WP_000615976.1 NZ_CP121151:4197352-4197630 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT275915 WP_000729704.1 NZ_CP121151:4197089-4197349 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References