Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 608818..609617 Replicon chromosome
Accession NZ_CP121149
Organism Escherichia coli strain E14

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag P1I99_RS02940 Protein ID WP_000347266.1
Coordinates 608818..609282 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag P1I99_RS02945 Protein ID WP_001307405.1
Coordinates 609282..609617 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P1I99_RS02910 (603820) 603820..604254 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
P1I99_RS02915 (604272) 604272..605150 - 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
P1I99_RS02920 (605140) 605140..605918 - 779 Protein_571 PTS N-acetylgalactosamine transporter subunit IIC -
P1I99_RS02925 (605929) 605929..606402 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
P1I99_RS02930 (606425) 606425..607705 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
P1I99_RS02935 (607954) 607954..608763 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
P1I99_RS02940 (608818) 608818..609282 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
P1I99_RS02945 (609282) 609282..609617 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
P1I99_RS02950 (609766) 609766..611337 - 1572 WP_001273741.1 galactarate dehydratase -
P1I99_RS02955 (611712) 611712..613046 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
P1I99_RS02960 (613062) 613062..613832 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T275878 WP_000347266.1 NZ_CP121149:c609282-608818 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT275878 WP_001307405.1 NZ_CP121149:c609617-609282 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References