Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2393714..2394276 Replicon chromosome
Accession NZ_CP121147
Organism Escherichia coli strain E20

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag P6H07_RS11490 Protein ID WP_000605676.1
Coordinates 2393998..2394276 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag P6H07_RS11485 Protein ID WP_000781370.1
Coordinates 2393714..2393998 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6H07_RS11470 (2389073) 2389073..2392120 + 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -
P6H07_RS11475 (2392133) 2392133..2393017 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
P6H07_RS11480 (2393010) 2393010..2393663 + 654 WP_000045650.1 formate dehydrogenase-N subunit gamma -
P6H07_RS11485 (2393714) 2393714..2393998 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
P6H07_RS11490 (2393998) 2393998..2394276 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
P6H07_RS11495 (2394462) 2394462..2395472 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
P6H07_RS11500 (2395606) 2395606..2397303 - 1698 WP_000433464.1 malate dehydrogenase -
P6H07_RS11505 (2397460) 2397460..2397597 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
P6H07_RS11510 (2397699) 2397699..2397914 - 216 WP_000495766.1 biofilm-dependent modulation protein -
P6H07_RS11515 (2398259) 2398259..2398690 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T275859 WP_000605676.1 NZ_CP121147:c2394276-2393998 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT275859 WP_000781370.1 NZ_CP121147:c2393998-2393714 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References