Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2500403..2500905 Replicon chromosome
Accession NC_017656
Organism Escherichia coli O55:H7 str. RM12579

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECO55CA74_RS12795 Protein ID WP_000767829.1
Coordinates 2500403..2500657 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECO55CA74_RS12800 Protein ID WP_001259255.1
Coordinates 2500654..2500905 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO55CA74_RS12770 2495418..2495645 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
ECO55CA74_RS12775 2495659..2496717 - 1059 WP_000492327.1 transport protein YeeE -
ECO55CA74_RS12780 2496896..2498254 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ECO55CA74_RS31115 2498244..2498306 - 63 WP_010723108.1 membrane protein YoeI -
ECO55CA74_RS12785 2498521..2499450 - 930 WP_000803334.1 LysR family transcriptional regulator -
ECO55CA74_RS12790 2499496..2500320 - 825 WP_000754737.1 SDR family oxidoreductase -
ECO55CA74_RS12795 2500403..2500657 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECO55CA74_RS12800 2500654..2500905 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECO55CA74_RS28675 2501189..2501239 + 51 WP_013009236.1 his operon leader peptide -
ECO55CA74_RS12805 2501385..2502284 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECO55CA74_RS12810 2502290..2503594 + 1305 WP_001419115.1 histidinol dehydrogenase -
ECO55CA74_RS12815 2503591..2504661 + 1071 WP_000109000.1 histidinol-phosphate transaminase -
ECO55CA74_RS12820 2504661..2505728 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T27584 WP_000767829.1 NC_017656:c2500657-2500403 [Escherichia coli O55:H7 str. RM12579]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T27584 NC_017656:c2500657-2500403 [Escherichia coli O55:H7 str. RM12579]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT27584 WP_001259255.1 NC_017656:c2500905-2500654 [Escherichia coli O55:H7 str. RM12579]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT27584 NC_017656:c2500905-2500654 [Escherichia coli O55:H7 str. RM12579]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References