Detailed information of TA system TA027570

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1725975..1726346
• Replicon: chromosome
• Accession: NC_017656
• Organism: Escherichia coli O55:H7 str. RM12579

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: ECO55CA74_RS08650
• Protein ID: WP_001443846.1
• Coordinates: 1726197..1726346 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1725975..1726153 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO55CA74_RS31845	1721727..1721897	+	171	WP_001625136.1	hypothetical protein	-
ECO55CA74_RS08625	1721930..1723303	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
ECO55CA74_RS08630	1723432..1724367	-	936	WP_000662472.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
ECO55CA74_RS28375	1724419..1725654	-	1236	WP_000040839.1	site-specific integrase	-
ECO55CA74_RS08640	1725656..1725871	-	216	WP_000079604.1	excisionase XisR	-
ECO55CA74_RS08645	1725971..1726159	-	189	WP_001302840.1	DUF1187 family protein	-
-	1725975..1726153	+	179	NuclAT_0	-	Antitoxin
-	1725975..1726153	+	179	NuclAT_0	-	Antitoxin
-	1725975..1726153	+	179	NuclAT_0	-	Antitoxin
-	1725975..1726153	+	179	NuclAT_0	-	Antitoxin
ECO55CA74_RS08650	1726197..1726346	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
ECO55CA74_RS08655	1726402..1727211	-	810	WP_000166313.1	recombination protein RecT	-
ECO55CA74_RS08660	1727204..1729876	-	2673	WP_000102126.1	exodeoxyribonuclease VIII	-
ECO55CA74_RS08665	1729969..1730157	-	189	WP_000199475.1	DUF1482 family protein	-
ECO55CA74_RS08670	1730154..1730342	-	189	WP_000449175.1	cell division inhibitor	-
ECO55CA74_RS08680	1730832..1730984	-	153	WP_000379610.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1724419..1768999	44580

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T27570 WP_001443846.1 NC_017656:c1726346-1726197 [Escherichia coli O55:H7 str. RM12579]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T27570 NC_017656:c1726346-1726197 [Escherichia coli O55:H7 str. RM12579]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT27570 NC_017656:1725975-1726153 [Escherichia coli O55:H7 str. RM12579]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin