Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 291099..291793 | Replicon | chromosome |
| Accession | NC_017656 | ||
| Organism | Escherichia coli O55:H7 str. RM12579 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | C3TNX7 |
| Locus tag | ECO55CA74_RS01360 | Protein ID | WP_001263495.1 |
| Coordinates | 291395..291793 (+) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1FJN6 |
| Locus tag | ECO55CA74_RS01355 | Protein ID | WP_000554757.1 |
| Coordinates | 291099..291392 (+) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ECO55CA74_RS01320 | 286377..286655 | - | 279 | WP_000615979.1 | type II toxin-antitoxin system mRNA interferase toxin YafQ | - |
| ECO55CA74_RS01325 | 286658..286918 | - | 261 | WP_000729705.1 | type II toxin-antitoxin system antitoxin DinJ | - |
| ECO55CA74_RS01330 | 287104..287877 | + | 774 | WP_000543891.1 | C40 family peptidase | - |
| ECO55CA74_RS30800 | 288053..288550 | + | 498 | Protein_251 | REP-associated tyrosine transposase RayT | - |
| ECO55CA74_RS01340 | 288868..289164 | - | 297 | Protein_252 | FHIPEP family type III secretion protein | - |
| ECO55CA74_RS01345 | 289136..289921 | + | 786 | WP_000207556.1 | putative lateral flagellar export/assembly protein LafU | - |
| ECO55CA74_RS01350 | 289992..291047 | + | 1056 | WP_001226186.1 | DNA polymerase IV | - |
| ECO55CA74_RS01355 | 291099..291392 | + | 294 | WP_000554757.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| ECO55CA74_RS01360 | 291395..291793 | + | 399 | WP_001263495.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| ECO55CA74_RS01365 | 291803..292255 | + | 453 | WP_001059871.1 | GNAT family N-acetyltransferase | - |
| ECO55CA74_RS01370 | 292574..292777 | + | 204 | Protein_258 | RNA ligase RtcB family protein | - |
| ECO55CA74_RS01375 | 292776..293297 | + | 522 | Protein_259 | peptide chain release factor H | - |
| ECO55CA74_RS01380 | 293354..294811 | - | 1458 | WP_001293009.1 | cytosol nonspecific dipeptidase | - |
| ECO55CA74_RS01385 | 295072..295530 | + | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| - | 296126..296206 | + | 81 | NuclAT_11 | - | - |
| - | 296126..296206 | + | 81 | NuclAT_11 | - | - |
| - | 296126..296206 | + | 81 | NuclAT_11 | - | - |
| - | 296126..296206 | + | 81 | NuclAT_11 | - | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15473.86 Da Isoelectric Point: 8.0949
>T27567 WP_001263495.1 NC_017656:291395-291793 [Escherichia coli O55:H7 str. RM12579]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T27567 NC_017656:291395-291793 [Escherichia coli O55:H7 str. RM12579]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.81 Da Isoelectric Point: 5.9050
>AT27567 WP_000554757.1 NC_017656:291099-291392 [Escherichia coli O55:H7 str. RM12579]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT27567 NC_017656:291099-291392 [Escherichia coli O55:H7 str. RM12579]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|