Detailed information of TA system TA027566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 286377..286918
• Replicon: chromosome
• Accession: NC_017656
• Organism: Escherichia coli O55:H7 str. RM12579

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: S1FHQ9
• Locus tag: ECO55CA74_RS01320
• Protein ID: WP_000615979.1
• Coordinates: 286377..286655 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: ECO55CA74_RS01325
• Protein ID: WP_000729705.1
• Coordinates: 286658..286918 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO55CA74_RS01305	284060..284638	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
ECO55CA74_RS01310	284743..285510	+	768	WP_001301698.1	class II glutamine amidotransferase	-
ECO55CA74_RS01315	285481..286221	-	741	WP_001225679.1	murein L,D-transpeptidase	-
ECO55CA74_RS01320	286377..286655	-	279	WP_000615979.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
ECO55CA74_RS01325	286658..286918	-	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
ECO55CA74_RS01330	287104..287877	+	774	WP_000543891.1	C40 family peptidase	-
ECO55CA74_RS30800	288053..288550	+	498	Protein_251	REP-associated tyrosine transposase RayT	-
ECO55CA74_RS01340	288868..289164	-	297	Protein_252	FHIPEP family type III secretion protein	-
ECO55CA74_RS01345	289136..289921	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
ECO55CA74_RS01350	289992..291047	+	1056	WP_001226186.1	DNA polymerase IV	-
ECO55CA74_RS01355	291099..291392	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	-
ECO55CA74_RS01360	291395..291793	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10830.62 Da
• Isoelectric Point: 10.0702

>T27566 WP_000615979.1 NC_017656:c286655-286377 [Escherichia coli O55:H7 str. RM12579]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T27566 NC_017656:c286655-286377 [Escherichia coli O55:H7 str. RM12579]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT27566 WP_000729705.1 NC_017656:c286918-286658 [Escherichia coli O55:H7 str. RM12579]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT27566 NC_017656:c286918-286658 [Escherichia coli O55:H7 str. RM12579]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Structures

Toxin

• AlphaFold DB: S1FHQ9