Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 679169..679731 Replicon chromosome
Accession NZ_CP121087
Organism Escherichia coli strain ST3

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag P5670_RS03440 Protein ID WP_000605676.1
Coordinates 679169..679447 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag P5670_RS03445 Protein ID WP_000781370.1
Coordinates 679447..679731 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5670_RS03415 (674755) 674755..675186 - 432 WP_000152305.1 peroxiredoxin OsmC -
P5670_RS03420 (675531) 675531..675746 + 216 WP_000495766.1 biofilm-dependent modulation protein -
P5670_RS03425 (675848) 675848..675985 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
P5670_RS03430 (676142) 676142..677839 + 1698 WP_000433464.1 malate dehydrogenase -
P5670_RS03435 (677973) 677973..678983 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
P5670_RS03440 (679169) 679169..679447 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
P5670_RS03445 (679447) 679447..679731 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
P5670_RS03450 (679959) 679959..680612 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
P5670_RS03455 (680605) 680605..681489 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
P5670_RS03460 (681502) 681502..684549 - 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T275658 WP_000605676.1 NZ_CP121087:679169-679447 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT275658 WP_000781370.1 NZ_CP121087:679447-679731 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References