Detailed information of TA system TA027560

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-rdlD/SymE(toxin)
• Location: 4972492..4972904
• Replicon: chromosome
• Accession: NC_017652
• Organism: Escherichia coli str. clone D i14

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: I14_RS24440
• Protein ID: WP_000132630.1
• Coordinates: 4972492..4972833 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	-
Coordinates	4972828..4972904 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I14_RS24425	4967561..4968841	-	1281	WP_001304526.1	DUF445 domain-containing protein	-
I14_RS24430	4969026..4969946	+	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
I14_RS27510	4970174..4970254	+	81	WP_020233658.1	hypothetical protein	-
I14_RS24435	4970352..4972445	+	2094	WP_000648234.1	DUF262 domain-containing protein	-
I14_RS24440	4972492..4972833	-	342	WP_000132630.1	endoribonuclease SymE	Toxin
-	4972828..4972904	+	77	NuclAT_6	-	Antitoxin
-	4972828..4972904	+	77	NuclAT_6	-	Antitoxin
-	4972828..4972904	+	77	NuclAT_6	-	Antitoxin
-	4972828..4972904	+	77	NuclAT_6	-	Antitoxin
-	4972828..4972904	+	77	NuclAT_7	-	Antitoxin
-	4972828..4972904	+	77	NuclAT_7	-	Antitoxin
-	4972828..4972904	+	77	NuclAT_7	-	Antitoxin
-	4972828..4972904	+	77	NuclAT_7	-	Antitoxin
I14_RS24445	4973054..4974823	-	1770	WP_000110076.1	restriction endonuclease subunit S	-
I14_RS24450	4974823..4976292	-	1470	WP_001315223.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T27560 WP_000132630.1 NC_017652:c4972833-4972492 [Escherichia coli str. 'clone D i14']
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T27560 NC_017652:c4972833-4972492 [Escherichia coli str. 'clone D i14']
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT27560 NC_017652:4972828-4972904 [Escherichia coli str. 'clone D i14']
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin