Detailed information of TA system TA027557

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4423574..4424176
• Replicon: chromosome
• Accession: NC_017652
• Organism: Escherichia coli str. clone D i14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I14_RS21810
• Protein ID: WP_000897302.1
• Coordinates: 4423574..4423885 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I14_RS21815
• Protein ID: WP_000356397.1
• Coordinates: 4423886..4424176 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I14_RS21785	4419487..4420086	+	600	WP_001443191.1	glucose-1-phosphatase	-
I14_RS21790	4420080..4420952	+	873	WP_000920762.1	virulence factor BrkB family protein	-
I14_RS21795	4420949..4421386	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I14_RS21800	4421431..4422372	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I14_RS21805	4422436..4423344	-	909	WP_001305059.1	alpha/beta hydrolase	-
I14_RS21810	4423574..4423885	+	312	WP_000897302.1	hypothetical protein	Toxin
I14_RS21815	4423886..4424176	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I14_RS21825	4424535..4424813	+	279	WP_001296612.1	hypothetical protein	-
I14_RS21830	4425209..4425427	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
I14_RS21835	4425643..4426572	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I14_RS21840	4426569..4427204	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I14_RS21845	4427201..4428103	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T27557 WP_000897302.1 NC_017652:4423574-4423885 [Escherichia coli str. 'clone D i14']
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T27557 NC_017652:4423574-4423885 [Escherichia coli str. 'clone D i14']
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT27557 WP_000356397.1 NC_017652:4423886-4424176 [Escherichia coli str. 'clone D i14']
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT27557 NC_017652:4423886-4424176 [Escherichia coli str. 'clone D i14']
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416