Detailed information of TA system TA027555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3585135..3585934
• Replicon: chromosome
• Accession: NC_017652
• Organism: Escherichia coli str. clone D i14

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: I14_RS17645
• Protein ID: WP_000347252.1
• Coordinates: 3585470..3585934 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: I14_RS17640
• Protein ID: WP_001296435.1
• Coordinates: 3585135..3585470 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I14_RS17625	3580920..3581690	-	771	WP_001058223.1	2-dehydro-3-deoxyglucarate aldolase	-
I14_RS17630	3581706..3583040	-	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
I14_RS17635	3583415..3584986	+	1572	WP_001273940.1	galactarate dehydratase	-
I14_RS17640	3585135..3585470	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
I14_RS17645	3585470..3585934	+	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
I14_RS17650	3585989..3586798	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
I14_RS17655	3587047..3588327	+	1281	WP_000681926.1	tagatose-bisphosphate aldolase subunit KbaZ	-
I14_RS17660	3588350..3588823	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
I14_RS17665	3588834..3589613	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
I14_RS17670	3589603..3590481	+	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
I14_RS17675	3590499..3590933	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T27555 WP_000347252.1 NC_017652:3585470-3585934 [Escherichia coli str. 'clone D i14']
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T27555 NC_017652:3585470-3585934 [Escherichia coli str. 'clone D i14']
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAGCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT27555 WP_001296435.1 NC_017652:3585135-3585470 [Escherichia coli str. 'clone D i14']
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT27555 NC_017652:3585135-3585470 [Escherichia coli str. 'clone D i14']
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5