Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3010137..3010864 Replicon chromosome
Accession NC_017652
Organism Escherichia coli str. clone D i14

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag I14_RS14870 Protein ID WP_000547555.1
Coordinates 3010553..3010864 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag I14_RS14865 Protein ID WP_000126293.1
Coordinates 3010137..3010556 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I14_RS14850 3006300..3008552 - 2253 WP_001107659.1 carbamoyltransferase HypF -
I14_RS14855 3008705..3009232 - 528 WP_001078777.1 electron transport protein HydN -
I14_RS14860 3009617..3010045 + 429 WP_000536064.1 hypothetical protein -
I14_RS14865 3010137..3010556 - 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
I14_RS14870 3010553..3010864 - 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
I14_RS14875 3011027..3011782 - 756 WP_001305268.1 hypothetical protein -
I14_RS14880 3011825..3012295 - 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
I14_RS14885 3012288..3012698 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
I14_RS14890 3012695..3013462 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
I14_RS14895 3013462..3014004 - 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
I14_RS14900 3014014..3015723 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T27552 WP_000547555.1 NC_017652:c3010864-3010553 [Escherichia coli str. 'clone D i14']
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T27552 NC_017652:c3010864-3010553 [Escherichia coli str. 'clone D i14']
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT27552 WP_000126293.1 NC_017652:c3010556-3010137 [Escherichia coli str. 'clone D i14']
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT27552 NC_017652:c3010556-3010137 [Escherichia coli str. 'clone D i14']
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCAGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATACCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References