Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2315827..2316329 Replicon chromosome
Accession NC_017652
Organism Escherichia coli str. clone D i14

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag I14_RS11695 Protein ID WP_000767822.1
Coordinates 2315827..2316081 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag I14_RS11700 Protein ID WP_001259255.1
Coordinates 2316078..2316329 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I14_RS11680 2312320..2313678 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
I14_RS27845 2313668..2313730 - 63 WP_010723108.1 membrane protein YoeI -
I14_RS11685 2313945..2314874 - 930 WP_000803366.1 LysR family transcriptional regulator -
I14_RS11690 2314920..2315744 - 825 WP_000754733.1 SDR family oxidoreductase -
I14_RS11695 2315827..2316081 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
I14_RS11700 2316078..2316329 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
I14_RS25995 2316612..2316662 + 51 WP_001364200.1 his operon leader peptide -
I14_RS11705 2316808..2317707 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
I14_RS11710 2317713..2319017 + 1305 WP_001297912.1 histidinol dehydrogenase -
I14_RS11715 2319014..2320084 + 1071 WP_000108995.1 histidinol-phosphate transaminase -
I14_RS11720 2320084..2321151 + 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T27551 WP_000767822.1 NC_017652:c2316081-2315827 [Escherichia coli str. 'clone D i14']
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T27551 NC_017652:c2316081-2315827 [Escherichia coli str. 'clone D i14']
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT27551 WP_001259255.1 NC_017652:c2316329-2316078 [Escherichia coli str. 'clone D i14']
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT27551 NC_017652:c2316329-2316078 [Escherichia coli str. 'clone D i14']
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References