275459

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4374203..4374897 Replicon chromosome
Accession NZ_CP120944
Organism Escherichia coli O157:H7 str. EDL933

Toxin (Protein)


Gene name yafO Uniprot ID C3TNX7
Locus tag P7249_RS22195 Protein ID WP_001263495.1
Coordinates 4374203..4374601 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag P7249_RS22200 Protein ID WP_000554757.1
Coordinates 4374604..4374897 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (4369790) 4369790..4369870 - 81 NuclAT_12 - -
- (4369790) 4369790..4369870 - 81 NuclAT_12 - -
- (4369790) 4369790..4369870 - 81 NuclAT_12 - -
- (4369790) 4369790..4369870 - 81 NuclAT_12 - -
P7249_RS22170 (4370466) 4370466..4370924 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
P7249_RS22175 (4371185) 4371185..4372642 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
P7249_RS22180 (4372699) 4372699..4373220 - 522 Protein_4348 peptide chain release factor H -
P7249_RS22185 (4373219) 4373219..4373422 - 204 Protein_4349 RtcB family protein -
P7249_RS22190 (4373741) 4373741..4374193 - 453 WP_001059871.1 GNAT family N-acetyltransferase -
P7249_RS22195 (4374203) 4374203..4374601 - 399 WP_001263495.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
P7249_RS22200 (4374604) 4374604..4374897 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
P7249_RS22205 (4374949) 4374949..4376004 - 1056 WP_001226188.1 DNA polymerase IV -
P7249_RS22210 (4376075) 4376075..4376860 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
P7249_RS22215 (4376832) 4376832..4378544 + 1713 Protein_4355 flagellar biosynthesis protein FlhA -
P7249_RS22220 (4378689) 4378689..4379186 - 498 Protein_4356 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15473.86 Da        Isoelectric Point: 8.0949

>T275459 WP_001263495.1 NZ_CP120944:c4374601-4374203 [Escherichia coli O157:H7 str. EDL933]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT275459 WP_000554757.1 NZ_CP120944:c4374897-4374604 [Escherichia coli O157:H7 str. EDL933]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References