Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 695876..696603 Replicon chromosome
Accession NZ_CP120944
Organism Escherichia coli O157:H7 str. EDL933

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag P7249_RS03475 Protein ID WP_000550189.1
Coordinates 695876..696190 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag P7249_RS03480 Protein ID WP_000560249.1
Coordinates 696187..696603 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P7249_RS03455 (692034) 692034..693020 - 987 WP_000617675.1 Gfo/Idh/MocA family oxidoreductase -
P7249_RS03460 (693099) 693099..693791 - 693 WP_000942543.1 vancomycin high temperature exclusion protein -
P7249_RS03465 (693868) 693868..694371 - 504 WP_001295542.1 M48 family metallopeptidase -
P7249_RS03470 (694456) 694456..695592 + 1137 WP_000018678.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
P7249_RS03475 (695876) 695876..696190 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
P7249_RS03480 (696187) 696187..696603 + 417 WP_000560249.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
P7249_RS03485 (696648) 696648..698666 - 2019 WP_000121479.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
P7249_RS03490 (699192) 699192..701543 - 2352 WP_000695517.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T275444 WP_000550189.1 NZ_CP120944:695876-696190 [Escherichia coli O157:H7 str. EDL933]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15016.48 Da        Isoelectric Point: 4.5805

>AT275444 WP_000560249.1 NZ_CP120944:696187-696603 [Escherichia coli O157:H7 str. EDL933]
MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References