275443

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 652926..653725 Replicon chromosome
Accession NZ_CP120944
Organism Escherichia coli O157:H7 str. EDL933

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag P7249_RS03250 Protein ID WP_000347273.1
Coordinates 652926..653390 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID C3ST72
Locus tag P7249_RS03255 Protein ID WP_001302819.1
Coordinates 653390..653725 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P7249_RS03220 (647927) 647927..648361 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
P7249_RS03225 (648379) 648379..649257 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
P7249_RS03230 (649247) 649247..650026 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
P7249_RS03235 (650037) 650037..650510 - 474 WP_001301475.1 PTS N-acetylgalactosamine transporter subunit IIB -
P7249_RS03240 (650533) 650533..651813 - 1281 WP_000681927.1 tagatose-bisphosphate aldolase subunit KbaZ -
P7249_RS03245 (652062) 652062..652871 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
P7249_RS03250 (652926) 652926..653390 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
P7249_RS03255 (653390) 653390..653725 - 336 WP_001302819.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
P7249_RS03260 (653874) 653874..655445 - 1572 WP_001273776.1 galactarate dehydratase -
P7249_RS03265 (655820) 655820..657154 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
P7249_RS03270 (657170) 657170..657940 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T275443 WP_000347273.1 NZ_CP120944:c653390-652926 [Escherichia coli O157:H7 str. EDL933]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12388.97 Da        Isoelectric Point: 4.8616

>AT275443 WP_001302819.1 NZ_CP120944:c653725-653390 [Escherichia coli O157:H7 str. EDL933]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure

References