Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4002909..4003506 Replicon chromosome
Accession NZ_CP120847
Organism Klebsiella pneumoniae strain KPHRJ

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag P5D18_RS19570 Protein ID WP_004142563.1
Coordinates 4003189..4003506 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag P5D18_RS19565 Protein ID WP_004142561.1
Coordinates 4002909..4003196 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5D18_RS19535 (3998989) 3998989..3999237 + 249 WP_020325243.1 DUF1158 domain-containing protein -
P5D18_RS19540 (3999255) 3999255..3999596 - 342 WP_025403994.1 RamA family antibiotic efflux transcriptional regulator -
P5D18_RS19545 (3999627) 3999627..4000742 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
P5D18_RS19550 (4000922) 4000922..4001503 + 582 WP_215402451.1 TetR/AcrR family transcriptional regulator -
P5D18_RS19555 (4001503) 4001503..4001871 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
P5D18_RS19560 (4001991) 4001991..4002644 + 654 WP_032419263.1 oxygen-insensitive NAD(P)H nitroreductase -
P5D18_RS19565 (4002909) 4002909..4003196 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
P5D18_RS19570 (4003189) 4003189..4003506 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
P5D18_RS19575 (4003691) 4003691..4004734 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
P5D18_RS19580 (4005404) 4005404..4006270 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
P5D18_RS19585 (4006379) 4006379..4007806 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T275423 WP_004142563.1 NZ_CP120847:c4003506-4003189 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT275423 WP_004142561.1 NZ_CP120847:c4003196-4002909 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References