Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4630671..4631350 Replicon chromosome
Accession NZ_CP120633
Organism Escherichia coli strain USVAST219

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag P6O79_RS22605 Protein ID WP_000057523.1
Coordinates 4630671..4630973 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag P6O79_RS22610 Protein ID WP_000806442.1
Coordinates 4631009..4631350 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P6O79_RS22580 (4626047) 4626047..4627699 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
P6O79_RS22585 (4627737) 4627737..4628240 - 504 WP_000667000.1 hypothetical protein -
P6O79_RS22590 (4628237) 4628237..4628893 - 657 WP_015674862.1 hypothetical protein -
P6O79_RS22595 (4629061) 4629061..4629540 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
P6O79_RS22600 (4629744) 4629744..4630538 - 795 WP_000365147.1 TraB/GumN family protein -
P6O79_RS22605 (4630671) 4630671..4630973 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
P6O79_RS22610 (4631009) 4631009..4631350 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
P6O79_RS22615 (4631408) 4631408..4633912 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
P6O79_RS22620 (4634174) 4634174..4635106 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T275310 WP_000057523.1 NZ_CP120633:4630671-4630973 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT275310 WP_000806442.1 NZ_CP120633:4631009-4631350 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References